FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16290755
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC54300.017.1037798
AAACCCC59650.015.6922699
TTAAACC113200.08.9675727
CTAGCAA71850.08.3165194
ATGTAGT140350.07.64134451
TGTAGTT174100.06.99040172
GTTCTAG101400.06.73712161
GTAGTTA165900.06.3792043
GTCTAGA35052.1827873E-116.28727671
GTTAAAC167100.06.247326
GTATAGA129250.06.19477221
GCAACAC107600.06.0218857
TTCTAGC119900.06.0061342
GTCCTAG15750.00141683955.94235133
GTCATTT159050.05.9116081
CAACACT111900.05.7904818
GTCTCTA57700.05.7288321
GTATATC169000.05.6504891
GTCTTTA198350.05.4809881
GTATATA202400.05.44389961
Produced by FastQC (version 0.11.5)