FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204414
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA28100.034.5889668
ACACTAT51350.020.0499789
CCACACT57650.017.234157
ACCACAC63800.015.7985596
AACCACA75800.012.7271415
CAACCAC80000.012.4202534
CTAGCTC17200.011.97173935-39
ATACAAC91350.011.6248761
ACAACCA101000.010.4794223
TACAACC103850.010.3304962
CCGTTAA63700.08.7034744
TCTAGCT25100.08.54795435-39
CGTTAAA66100.08.4954915
GTTAAAT74650.07.8120556
CTCTAGC28400.07.60540835-39
TCTCTAG29800.07.248108435-39
TTAGGGG33350.06.8219094105-109
CCCCTAA32150.06.7630095110-114
TCCGTTA89800.06.65492443
CTATAGC36200.06.126597410-14
Produced by FastQC (version 0.11.5)