FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204414
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA26550.031.4536888
ACACTAT51900.016.2289989
CCACACT54400.015.6161067
ACCACAC62100.014.1435316
AACCACA71350.011.9067745
CAACCAC80600.011.1661244
ATACAAC92400.010.6024251
CTAGCTC17600.09.57073935-39
CCGTTAA66450.09.5348784
CGTTAAA67450.09.2863155
GTTAAAT70600.09.1775656
TACAACC98300.09.0886982
ACAACCA99700.08.8105633
TCCGTTA86950.07.61828473
TCTAGCT25400.06.9717835-39
CCCCTAA31750.06.1675196110-114
CTCTAGC30950.05.67507335-39
TTAAATT132000.05.6721687
GTTCCGT144450.05.41551
TCTCTAG33200.05.377196335-39
Produced by FastQC (version 0.11.5)