FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16321615
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACT61300.08.0143412
TTGCGCG118350.07.96856937
GTTGCGC133950.07.25551756
CCCACTT79200.06.8189253
TTAGTCC71700.06.42596249
CACCCAC72750.06.41610241
CGTTGCG153350.06.29069145
CCGTTGC159800.06.2624364
CGTTAAC32300.05.9734583120-124
GCGCGCT161000.05.72349749
CTGCCGT199450.05.33879381
TGCGCGC186950.05.23709878
TTTAGTC93650.05.22731268
TCTAGCC24002.8560727E-45.09935245
Produced by FastQC (version 0.11.5)