FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16321615
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACT63600.010.868032
CACCCAC74400.010.0204251
TTAGTCC71100.09.7214649
CCCACTT84150.08.8981823
TTTAGTC96850.07.73158
TTGCGCG121800.07.27089557
GTTGCGC133650.06.73368026
CACTTTA112700.06.3883135
CCACTTT117300.06.260724
CGTTGCG152400.05.95244935
CCGTTGC156500.05.61264474
GCGCGCT167950.05.3587089
Produced by FastQC (version 0.11.5)