FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12549166
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA16400.039.7415051
CTAGAAC16800.039.427153
TCTAGAA22950.029.8230482
TAGAACC48100.014.817414
CTAGGTC9750.08.713115135-139
GCTAGTC10600.08.69460175-79
CCGGTCC79250.08.4488069
GCTAGGT10150.08.369741135-139
CTAGTCC10950.08.15366975-79
GGCTAGT11200.07.971668775-79
CTAGCTA11150.07.877586130-134
AGCTAGG12250.07.5226345135-139
ACCGGTC97600.07.2291618
GAGCTAG13200.06.981532650-54
CGCTAGC13100.06.9248033130-134
AGGCTAG14650.06.880752675-79
TCGCTAG13150.06.679474130-134
TAGCTAG10450.06.6137996135-139
AACCGGT112100.06.29407747
GCTAGCT14850.06.1087484130-134
Produced by FastQC (version 0.11.5)