FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18185622
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC464200.2552565977671811No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT299950.16493799332241701TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA220400.024.1386839
AGAGCAC235200.022.5585378
GATCGGA256100.020.545121
TCGGAAG316600.016.9082683
ATCGGAA337450.016.1197662
AAGAGCA342200.015.56798657
GAAGAGC356700.014.93514256
GGAAGAG421400.012.8299865
CGGAAGA455050.012.0258684
GAGTCAA105000.010.0653335-39
AGAGTCA111600.09.88411830-34
AGTCACA119150.09.26903525-29
CAGAGTC123100.08.99583830-34
AGTGAGT54700.08.3241541
ACAGAGT135350.08.08590630-34
GTCACAG145300.07.739601625-29
CACAGAG148500.07.525036330-34
CCAGTCA157750.07.22005225-29
GTGAGTA56500.07.1410292
CAGTCAC161150.07.112396225-29
Produced by FastQC (version 0.11.5)