FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9959539
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT31750.035.4712871
GTGAGTG36350.031.1048362
ACTAATG18500.031.0273251
CTAATGC20300.028.3809322
CCACTGT44500.024.3284931
TGAGTGC50200.022.9468153
CACTGTG52850.021.393772
ACACACT33150.019.9865112
GTGCCTA21400.019.518522
ACTGTGC63550.018.0130713
GAGTGCA65650.017.9837554
CAATGCA86750.017.176695
ACACTGT40650.015.0532284
AACACAC44200.014.466031
AGTGCAG84600.014.0395165
TCAATGC115400.013.1004074
TAATGCA48100.012.7227213
ATGCAGG183950.012.6395157
ACGATTG58400.011.7150312
AATGCAG131700.011.5871796
Produced by FastQC (version 0.11.5)