FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12234870
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTG63400.019.1917239
CTGAGTT47400.013.518278
TCTGAGT34250.011.7717827
TTTTTTT476300.07.99608475
GTGAGTT58750.07.96553478
CTCTGAG30550.07.3057576
TAGTGAG20851.3278623E-87.2515026
GGGGGGG69750.06.91793872
CTGTAGT36009.094947E-126.2418921
TAGTCTG37602.2919266E-105.7443994
AGTGAGT29001.8610626E-75.7101117
ACTGAGT25552.3334165E-55.3539887
GTAGTCT40801.886292E-95.2938583
GTCTGAG37303.6956408E-85.21158366
Produced by FastQC (version 0.11.5)