FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14389594
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC12356.366463E-1111.0789893
CTCTAGA10457.659946E-810.3449821
AGTGGTA57600.010.249745
TCTAGAA17705.456968E-108.5439362
CTGAGAG56400.08.0503771
CAGTGGT75800.07.694274
TGAGAGG56050.07.4518542
AGCAGTG95400.06.56723982
GTGGTAT96150.06.5146546
GTATCAA99850.06.0569319
GGTATCA104900.05.7653448
TGGTATC107050.05.11157
AAGCAGT127750.05.0773411
Produced by FastQC (version 0.11.5)