FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11632586
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT38650.020.1110461
GTGAGTG39150.019.8673862
TGAGTGC55950.014.2858553
GAGTGCA59250.013.729184
CACTGTG48300.012.8233072
CCACTGT47000.012.6740771
ACTAATG36150.011.4542571
ACTGTGC62800.010.8929973
AGTGCAG78200.010.2178075
CTAATGC39700.09.9774822
ACACACT36000.09.8026232
GTGCCTA29900.08.9120852
ACACTGT47200.08.540844
AACACAC49850.08.4520721
ATGCAGG185700.08.2566097
CAATGCA113650.07.98072965
GTGCAGG175250.07.9274466
CTGTGCA104100.06.5694264
TCAATGC160200.06.5156844
CCATTTG110500.06.3111621
Produced by FastQC (version 0.11.5)