FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16472448
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTTCTAATCTCGTAT191550.11628508403851084TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT45800.019.4906648
AACACTA47050.019.278867
CACTATA50450.017.837119
AAACACT79800.011.7276486
ACCAAAC98750.09.6997173
CCAAACA102950.09.0236614
CAAACAC109250.08.829865
TACCAAA142800.06.8587652
GAGCACA489750.06.5265589
ATACCAA170050.06.11410571
AGAGCAC521400.06.0750818
CTATAGC39300.05.935588410-14
CACGTGT167150.05.538933140-144
TCACGTG179850.05.500065140-144
AGTCACG194650.05.4221444140-144
AAGAGCA612550.05.3591237
CACACGT379500.05.353487140-144
CACGTCT360550.05.299403140-144
ACTATAG40300.05.216618510-14
ACACGTC388550.05.1620917140-144
Produced by FastQC (version 0.11.5)