FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16472448
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT186480.11320721728792223Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA46500.014.2478577
ACACTAT48100.013.7739148
CACTATA50150.013.4980679
AAACACT79300.08.8995396
ACCAAAC96600.07.9007483
CCAAACA101850.07.49317464
CAAACAC109150.06.99345065
GTGTTAG157500.06.042946140-144
GTTAGAC148750.06.020898140-144
TACCAAA138550.05.87227152
TTAGACG142850.05.765588140-144
AGAGCGT566800.05.5522148
CGTATCA123200.05.540076360-64
TGTTAGA174750.05.4711514140-144
TAGATCG225500.05.395578140-144
TAGATCT119700.05.38920740-44
AGTGTTA184650.05.372763140-144
AAGAGCG632450.05.1808367
CTAGATC56300.05.1661954140-144
TCATTAA136350.05.16405465-69
Produced by FastQC (version 0.11.5)