FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16636516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT48050.013.34380151
TAACCCC49850.013.1313959
GTGAGTA49300.012.8522892
ATAACCC89150.07.4233718
GTCTCAT59550.06.8516721
GTATAAC118850.05.99665266
GAGTATA114350.05.91873074
TGAGTAT123650.05.7064873
TATAACC130700.05.5599797
Produced by FastQC (version 0.11.5)