FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14268353
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAG63550.010.9883687
AAGGTAC69300.010.9161922
TACACAA64650.010.5783616
GTACACA65550.010.5430175
AGGTACA80150.08.6281423
GGTACAC82700.08.2750814
CTAACAC21200.07.334577655-59
AAAGGTA121950.06.6655371
GCTAACA30850.06.020658550-54
CACAAGG137800.05.95569758
TGCTAAC29700.05.91442950-54
GGGTTAG15050.00555972485.7399496
TCACTAA34750.05.0549045115-119
Produced by FastQC (version 0.11.5)