FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087548
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA158850.011.9952271
GAGCACA163000.011.5713589
AGAGCAC168600.010.9735258
TCGGAAG190600.09.97933
ATCGGAA220900.08.7736132
AAGAGCA245500.07.7414637
GAAGAGC272600.07.1566256
CCATGAT187800.07.05984931
CCATGGA141600.06.80500271
CCATGGC244050.06.5013751
CCATGTC132850.06.48971131
CCATGCG194650.06.47641661
CCATGAA168600.06.4457521
GGAAGAG307950.06.42861375
CGGAAGA309000.06.38700634
CCATGAG104550.06.30604841
CCATGAC157400.06.21396731
CCATGGT129500.06.2100221
CCTGACG165000.06.19120361
CCATGCA143800.05.99553631
Produced by FastQC (version 0.11.5)