FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087548
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA176300.010.6135381
TCGGAAG201850.09.1660833
AGAGCGT206000.08.8765558
ATCGGAA230000.08.4564112
GAGCGTC223900.08.2312149
AAGAGCG242300.07.81412467
CCATGAT186050.07.15013461
CCATGCG191950.07.08267551
CCATGAG102950.07.02880241
CCATGAA166300.06.98839431
GAAGAGC282350.06.80776176
CCATGTG125650.06.340691
AACACTA52400.06.31981757
CGGAAGA313950.06.2371954
GGAAGAG310250.06.2187615
ACACTAT55600.06.2150478
CCATACC112700.06.1612981
CCATGCT175550.06.1205291
CCATGGT123300.06.1058571
CCTGACG165100.06.06521221
Produced by FastQC (version 0.11.5)