FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18277235
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT197670.10815093202007851TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA234200.014.5210981
AGAGCAC277950.012.58579358
GAGCACA280300.012.4806519
TCGGAAG287200.012.0884213
ATCGGAA307500.011.3143072
AAGAGCA383800.09.2087737
CGGAAGA406200.08.6179374
GGAAGAG420700.08.4866135
GAAGAGC505600.07.189686
CAGTCAC118500.06.07724325-29
AGTCACG131150.05.644815425-29
CCAGTCA138150.05.463051325-29
CTCGTAT149350.05.17678340-44
TCTCGTA153150.05.057736440-44
TCGTATG153550.05.034900745-49
Produced by FastQC (version 0.11.5)