FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18277235
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT200860.10989627260359677Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA235150.014.262311
TCGGAAG288150.011.8239643
ATCGGAA309850.011.137412
AGAGCGT329800.010.6086798
TAGATCT71950.09.80581840-44
AAGAGCG392600.09.0033837
GAGCGTC395100.08.964669
CGGAAGA405650.08.7010864
GGAAGAG424000.08.3191895
AGATCTC88350.08.18079945-49
AGTGTAG93850.07.517618740-44
GAAGAGC504100.07.3542986
GTAGATC102550.07.00621440-44
CAGTGTA108350.06.724549335-39
TCAGTGT118300.06.195475635-39
GAGTGTT114700.06.16330325-29
GGGGGGG135650.05.99698737
AGTGTTC123950.05.796281325-29
CATTAAA132800.05.778714765-69
TCATTAA130300.05.723839865-69
Produced by FastQC (version 0.11.5)