FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12102951
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAA51150.014.9212081
CTAGCGT10300.014.53978690-94
GCTAGCG12650.012.18030285-89
TGCTAGC15250.010.19808785-89
AATTCTC80750.010.0754288
ATGCTAG16200.09.86671985-89
CTAAAAA96750.08.1859552
TTAGGGG19000.07.95787160-64
GCGCTAA21450.07.5188656110-114
ATTCTCC124750.06.63729
AAATTCT135650.06.3161847
TAGGGGG26850.06.274842360-64
AAAATTC142250.06.0231316
TTTAGGG27550.05.95856455-59
ACTAATG29700.05.575731880-84
CGCTAAA34600.05.160738115-119
CTAATGC35800.05.027909380-84
CTGCTAA38250.05.0070233130-134
Produced by FastQC (version 0.11.5)