FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16457880
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA126400.020.5580799
AGAGCAC140650.018.6285938
GATCGGA153800.017.4283241
AACACTA65850.016.3972027
ACACTAT69100.015.83375558
TCGGAAG210000.013.0001943
CACTATA83200.012.8909629
AAGAGCA221000.012.2144317
ATCGGAA235200.011.6384032
AAACACT105400.010.4492496
CAAACAC101200.010.3138715
GGAAGAG265850.010.2350365
GAAGAGC269300.010.2108356
CGGAAGA305800.09.2573144
GCCCTTA61550.09.1284374
TAGCCCT69200.08.1196182
AGTCACG81900.06.96358825-29
TAACAAA181200.06.718552
ATAACAA201650.06.42962931
CAGTCAC90800.06.32861825-29
Produced by FastQC (version 0.11.5)