FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16816528
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT373920.22235267589124225Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA287650.019.936861
GAGCGTC296750.019.676169
AGAGCGT326500.017.9053618
TCGGAAG349200.016.7417053
AAGAGCG383600.015.409017
ATCGGAA410400.014.3561162
CGGAAGA438600.013.5590534
GAAGAGC462900.012.8470396
GGAAGAG493100.012.2203565
TAGATCT123050.010.1569840-44
GTAGATC149200.08.65665540-44
TAGTGTA143950.08.56202435-39
AGATCTC151750.08.16033745-49
AGTGTAG152850.07.97892640-44
GATCTCG169450.07.75831845-49
GTGGTCG176800.07.50962150-54
ATAGTGT175400.07.223828335-39
TGGTCGC184550.07.209867550-54
CGCCGTA199300.06.777455-59
GTGTAGA197200.06.76860740-44
Produced by FastQC (version 0.11.5)