FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889157
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGACCATCTCGTAT350670.22069767452105862TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA224450.028.866049
AGAGCAC241000.026.8240038
GATCGGA269350.024.5039751
TCGGAAG294050.022.2737793
AAGAGCA316750.020.8863817
ATCGGAA330050.020.3464582
GAAGAGC356900.018.5367366
GGAAGAG361550.018.2187425
CGGAAGA425450.015.6953744
AGTCACA114150.011.352077525-29
ACACACG115400.011.24141330-34
CACACAC120650.010.668715530-34
CAGTCAC124200.010.60738425-29
CTCGTAT121300.010.58857940-44
GTCACAC125650.010.3130925-29
CACACGA131300.010.18715630-34
TCACACA126950.010.16195530-34
TCTCGTA131300.09.92470340-44
CCAGTCA140600.09.6056425-29
TCGTATG136600.09.54965445-49
Produced by FastQC (version 0.11.5)