FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889157
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATGTTTGTGTAGATCT393640.24774127412801067Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA276200.023.7948651
AGAGCGT301200.021.6537698
TCGGAAG304750.021.2838843
ATCGGAA339000.019.6352372
AAGAGCG332900.019.6134367
GAAGAGC360950.018.1495366
GGAAGAG369300.017.778215
GAGCGTC372850.017.5505189
CGGAAGA433200.015.4216944
TAGATCT110100.012.04434940-44
TGTGTAG113200.011.67635440-44
AGTGTCG121600.010.82191125-29
GAGTGTC127100.010.28564625-29
AGAGTGT132900.010.15093225-29
GTAGATC146900.09.34075740-44
AGATCTC147900.09.20976345-49
TTGTGTA147550.09.17282235-39
GTGTAGA151500.09.11416640-44
GTAGGGA151600.08.94648715-19
TAGGGAA152650.08.86608615-19
Produced by FastQC (version 0.11.5)