FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13832404
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA57250.019.7423529
GCCACTC67550.017.0517678
AGCCACT89800.012.906977
AAGCCAC97650.012.0168356
CGTTAAA67500.09.172185
GGCCTAA27450.09.12838575-79
ACAAAGC144350.08.7844183
CCGTTAA60450.07.9814834
GTTAAAT93800.07.21439276
TACAAAG195650.06.88608842
GCCCCTA36550.06.7367706110-114
GGCCCCT38000.06.631283110-114
TGGCCCC40050.06.36376110-114
CTCACTA42600.06.049768415-19
AAAGCCA219300.06.0074535
CAAAGCC212900.05.91926054
CCTAGAC12550.05.8516746120-124
ATACAAA249700.05.76043561
ACACACT46200.05.57844350-54
CTAGACC15300.05.3645973120-124
Produced by FastQC (version 0.11.5)