FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16995310
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA79250.022.984767
ACACTAT82900.021.7990688
CACTATA84000.021.511459
AAACACT113600.015.9713246
CAAACAC134700.013.8436585
ACCAAAC141850.012.7909853
CCAAACA146100.012.3196844
AGAGCAC192350.09.2453538
GAGCACA201900.08.6645359
GATCGGA206050.08.5484931
TACCAAA228650.08.387012
ATACCAA292100.07.1265821
GGCCCCT58500.07.113243685-89
TCGGAAG273300.06.6915523
TTTAGGC65450.06.534627460-64
ATCGGAA299000.06.5101242
TGGCCCC65850.06.188086585-89
TACACTC66300.06.0806465115-119
GGCCCCA68700.05.9944545-49
AAGAGCA316200.05.96565537
Produced by FastQC (version 0.11.5)