FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13090776
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG408230.3118455315406818No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA315960.24136078716800288No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA230000.17569623068945647No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGCCGGTGTAGATCT229330.17518441993049153Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA247000.014.7346231
GAGCGTC265000.013.93604859
AGAGCGT286900.012.9475418
TCGGAAG291900.012.77547553
ATCGGAA325150.011.6086372
AAGAGCG339050.011.0409837
CGGAAGA357650.010.6683944
GAAGAGC413500.09.2970586
GGAAGAG435050.08.8696615
TAGATCT106400.07.375134540-44
AGATCTC119950.06.614170645-49
GTAGATC128650.06.233909640-44
TCCCTAC43250.06.1586259
AATCCCC77200.06.1545299
TGGTCGC139800.05.973774450-54
GTGGTCG143650.05.86378850-54
TAGCCGG134950.05.71877535-39
CGCCGTA150400.05.68677855-59
GATCTCG146950.05.634070445-49
CGTATCA153250.05.599581260-64
Produced by FastQC (version 0.11.5)