FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17951511
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA25900.014.2732441
GTCTCAG53250.09.8580531
AACACTA67700.07.4438679
TTAGACC23754.0199666E-107.2780667
CAACACT75350.07.07325368
CCCTATA52900.06.80672033
ACACTAG18450.06.242553770-74
TAGTTAG26151.4984653E-76.05925664
TCCCTAT73100.05.7135862
GTTAGAC27804.9222945E-75.6996246
TAGACCC38551.4313628E-85.23123748
AGTTAGA33305.299771E-75.1908135
CAACTCT75550.05.1457489
Produced by FastQC (version 0.11.5)