FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13903025
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC42250.015.8584631
GTATATT246050.010.9217021
GTATAGA96950.09.3632711
TGTGTCA69450.09.0248052
GTCTCTA51900.09.0229981
GTATATC155150.08.4513191
GTATTGA186650.08.33739951
GTGTCAT74800.08.2833
GTATTAA182900.08.2326091
GTGTTGA102050.07.9069661
GTATTGG105200.07.73869281
GTATAGG53400.07.5552991
GTATTTA202850.07.458461
GTATAAG89250.07.34578231
GTATATA168750.07.34327131
GTATGTT110100.07.2634091
GTTAGCG21001.50485E-87.20451741
GTATTTG187300.07.15451241
GTATTAG66100.07.08462331
GTATAAA174550.07.05799151
Produced by FastQC (version 0.11.5)