FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13903025
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC41300.013.7727011
GTATATC155050.09.148221
GTATAGG52150.08.9743131
GTATAGA100850.08.9243431
GTATTGA187300.08.918511
GTATACG42400.08.8303841
GTATATT241750.08.4585121
GTATAAA174600.08.165131
GTCTAAA51050.08.0393551
GTGTCTG74000.07.8812561
GTATAAC79650.07.8645811
GTATTAA191200.07.832811
GTATATA167650.07.6876151
TGTGTCA72350.07.66292052
GTATAGC66150.07.6192171
GTATTAG69800.07.3239451
GTATTGG107250.07.25051071
GTATAGT62650.07.12545731
GTATTTA211050.07.06199031
GTATTTG184050.06.96347951
Produced by FastQC (version 0.11.5)