FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622528
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC13300.026.5263163
CTCTAGA14550.025.2371121
TCTAGAA23400.016.02
TAGAACC46800.07.53846174
CTAGACC9850.06.4324875120-124
CCTAGAC10500.06.0342855120-124
ACCACTA48800.05.9016398
TAGCGTA31057.302988E-75.33333353
CCACTAC60850.05.32456839
CTAGATC11950.05.3020926140-144
ACAACAC83150.05.282023
ATCCTAG15200.05.210526115-119
CACCACT67600.05.0059177
Produced by FastQC (version 0.11.5)