FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139150
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT33650.018.4011887
AACACTA39850.015.5382686
CACTATA41650.014.52100858
ACTATAG48200.012.8464739
CAAACAC53950.011.7442074
AAACACT61700.011.0858995
CACACGT63250.010.29059315-19
CAGTCAC57750.09.47532530-34
CTCGGAA65400.09.3577999
AGCACAC76150.08.88772115-19
CACGTCT65250.08.496551520-24
CGTCTGA82050.08.2837320-24
TACAAAC80000.08.1900012
GCACACG86950.08.09844715-19
CCAGTCA77250.07.95961230-34
ACGTCTG86600.07.599076320-24
ACAAACA89850.07.05175263
GAGCACA86700.06.9425615-19
GTCTGAA111750.06.262550420-24
TGAACTC112050.06.258634625-29
Produced by FastQC (version 0.11.5)