FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507068
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT428080.2950837481426295TruSeq Adapter, Index 10 (97% over 37bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC202050.13927693728326082No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG167860.11570911503275506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT139200.052.804614
AGTGGTA139750.052.647045
AGCAGTG142050.051.491772
GCAGTGG152900.048.026183
GTGGTAT184550.040.1789056
AAGCAGT206350.035.4612541
GGTATCA216700.034.4836588
TGGTATC220100.033.820147
GTATCAA226350.033.045329
GAGCACA478500.015.3760179
AGAGCAC481750.015.2573448
GATCGGA550450.013.2540171
TCGGAAG579550.012.6208383
AAGAGCA630500.011.8176247
ATCGGAA628950.011.7783562
CAACGCA135350.011.59507910-14
GAAGAGC647050.011.5376096
CGGAAGA640200.011.4814174
GGAAGAG712850.010.37163455
TCAACGC155150.010.11533410-14
Produced by FastQC (version 0.11.5)