FastQCFastQC Report
Sat 11 May 2024
EGAF00005187426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507068
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT416420.28704628667901744Illumina Single End PCR Primer 1 (97% over 34bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG209240.14423314207943327No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC172640.11900406064133703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)