FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12431487
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG42750.031.4562821
GAGTGAT46700.028.830453
TGAGTGA49650.028.5728822
AGTGATG71350.019.37594
ATGGTTG78300.017.7447478
GATGGTT92100.015.1640897
TGGTTGA98900.014.1942549
TAGTGTG26050.010.61192715-19
GTAGTGT34150.08.47434515-19
TGATGGT184700.08.2238966
GTGATGG187450.08.21882255
AGTGTGG63000.05.073564515-19
GGTAGTG61850.05.028226415-19
Produced by FastQC (version 0.11.5)