FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15702149
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA40650.014.8857242
AGTGAGT43300.013.9625161
TAAGGGG50100.012.7872059
GAGCACA192900.011.8290649
AGAGCAC200250.011.3226728
GATCGGA234300.010.1970621
TCGGAAG285000.08.3661583
ATCGGAA303450.07.97633462
GAAGAGC307400.07.75086646
GGAAGAG351250.07.07015375
ATAAGGG100050.06.90661148
AAGAGCA363300.06.5384487
CGGAAGA400950.06.28811934
GAGTATA148400.05.2909734
TAGATCG64150.05.1392293140-144
CTCGTAT100650.05.0926440-44
TCTCGTA106550.05.05387940-44
Produced by FastQC (version 0.11.5)