FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15505687
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA51850.020.8261837
ACACTAT52400.020.6075888
CACTATA58000.018.8661299
AAACACT86750.012.86241156
CAAACAC100450.011.036495
TCAAACA137150.08.5617054
ATCAAAC143250.08.0461253
GATCGGA152550.07.7775911
TATTTAG63750.07.5659229
GCCCTTA64900.07.3260134
TAGCCCT70700.06.72620442
AGAGCGT187000.06.65996178
ATATCAA220750.06.3368721
CACTCTA41350.06.05858575-79
CTATAGT43100.05.94619810-14
ATCGGAA224850.05.8961772
TCGGAAG209250.05.8869743
ACTATAG43800.05.818295510-14
TATCAAA226900.05.7793962
AGTACTA48000.05.60913530-34
Produced by FastQC (version 0.11.5)