FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14303130
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA64750.020.4587176
ACTATAG69900.019.4670529
ACACTAT71950.018.7116097
CACTATA73800.018.1449998
CAAACAC100900.013.986414
AAACACT117400.011.8969055
TAGCCCT57950.011.68127252
GCCCTTA63050.010.163574
TACAAAC152100.09.2325392
GTAGCCC76600.08.6356081
ACAAACA178550.08.3473543
AGCCCTT93500.07.7776443
TATTTAG111200.06.60405879
ATACAAA232350.06.3821611
GGCCCCA48500.06.1751745-49
CCCCTCA53200.05.92740285-89
CCCTTAT119450.05.9066375
AGGCCCC51050.05.61286645-49
GGCCCCT53500.05.544281585-89
TGAGGCC56300.05.39636240-44
Produced by FastQC (version 0.11.5)