FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9930469
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA28250.016.817119
GCCACTC42900.010.5712588
AGCCACT51200.09.7011347
AAGCCAC60900.08.1559626
GGCCTAA13750.07.645062475-79
CCCTTAA32400.07.10950854
CTCACTA16100.06.706157715-19
TACAAAG82000.06.6742552
ACAAAGC81550.06.0027193
GTGCCTA22351.5824848E-55.7995992
GTTCCCT61950.05.4988681
Produced by FastQC (version 0.11.5)