FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9930469
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA29150.014.0785939
CCCTTAA35450.09.14145854
GCCACTC44900.08.8197178
AGCCACT53450.08.0824287
GGCCTAA12150.07.22846775-79
AAGCCAC65300.06.50544746
CTTAAAT61250.06.11273436
ACAAAGC78700.05.948693
CCTTAAA60550.05.9459685
GTTCCCT60150.05.8995831
TCCCTTA58850.05.75220163
TACAAAG86150.05.2696042
Produced by FastQC (version 0.11.5)