FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14653106
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCC195950.133725914492122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT91850.031.116448
CACTATA91550.030.8249139
CAAACAC97500.029.5347945
AACACTA99750.028.5077387
AAACACT130850.022.8324726
TCAAACA159800.018.336024
ATCAAAC208350.014.4837133
GTATCCC119350.013.7361661
CCTTATT153400.012.1549446
CACCCTA52000.011.96155175-79
CCCTTAT153900.011.4605655
GTCTAGT12851.3278623E-1010.6445925
CCCCTCA61300.010.5229985-89
TATTTAG175850.09.988999
AGTACAC68400.09.64204110-114
TGAGGCC67650.09.64168440-44
GGCCCCA65900.09.63559145-49
TATCCCT192000.09.2652072
TACACTC68750.09.070516115-119
AGGCCCC71450.08.82667345-49
Produced by FastQC (version 0.11.5)