FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13542606
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAGG47850.010.8363032
CTAGAAC18450.010.1486713
CTGAGAG51600.09.9173091
CTCTAGA21750.08.6158721
GAGAGGC66350.08.2490673
CAGTGGT71000.07.80906064
AGTGGTA80300.06.99354555
AGAGGCT83700.06.36608844
AGCAGTG102300.06.0541532
GTGGTAT115250.05.6223776
TCTAGAA34706.866685E-95.6035712
CTGGTCT100500.05.1580419
CACACGT78300.05.1126432140-144
Produced by FastQC (version 0.11.5)