FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15923059
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA79250.029.5260057
ACACTAT78800.029.5118838
CACTATA81550.028.2518319
CAAACAC124400.019.4459255
AAACACT126850.018.8433156
GCCCTTA78250.017.2060284
TAGCCCT79300.016.8879412
TCAAACA175250.013.8450724
GTAGCCC103250.013.6028821
TATTTAG109700.012.7981919
ATCAAAC196000.012.4894933
CCCTAAG25550.011.2714246
CCCTTAT125950.010.7465575
AGCCCTT136900.010.6235563
CCTTATT160200.08.7635846
ATATCAA328200.08.0514391
CCCCTCA67250.08.05070585-89
TATCAAA343450.07.714742
GTCCCTA22551.3096724E-107.6627853
TTAGGCA71300.07.472540460-64
Produced by FastQC (version 0.11.5)