FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16755217
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGA86300.010.1304631
CTTGGCA181550.08.7080781
CTTGTGA77800.07.5851511
CTTGGGG100300.07.3363371
CTTGCGA87750.07.3062471
CTTGGTA82100.07.27661751
CTTGGTG145150.07.1775761
CTTGGGC126900.07.11899851
CTTGGCG258000.06.8901541
CTTGCTA37800.06.74582531
CTTGAGA77700.06.28221271
CTTGTGT67200.06.07124231
AACACTA36952.3646862E-116.0362547
CTTGGGT51100.05.84550141
GTTGGGA87600.05.8217391
CTTCCGA148100.05.80478051
CTTGGAG109500.05.78847271
CTTGTGG125600.05.74254471
CTTGCCA174550.05.6347251
GGTGGGA95850.05.6246881
Produced by FastQC (version 0.11.5)