FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15824772
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAA160500.09.1436931
GTTCCAA97250.07.69472171
GTACGAA80800.07.55289131
GGTCGAA164800.07.4944031
GTACTAA33700.07.32983731
GTCACAA65800.07.2872451
GTTCGAA94250.07.2458911
GTTAGAA40900.07.1052931
GTTACAA79500.06.9453121
GTACCAA62950.06.92469741
GTTATAA109900.06.7429171
GTTAGAC16303.938284E-56.68573141
CTTACAA82300.06.44418951
CTAGTAA21451.0346685E-66.4353421
GTCCCAA59600.06.33873221
CTAGCAA17501.05190855E-46.22728161
CCTTGAA202700.06.12897251
ATCGAAT124600.06.06643962
TCGAATC88000.05.97170933
CTTCGAA104700.05.9675641
Produced by FastQC (version 0.11.5)