FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17150565
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT76500.07.48907471
GTGTACG79450.07.39356041
GTGTATA66300.07.2192641
GTATACA70600.06.9850051
GTGTACA60300.06.8551991
CACTATA60150.06.7008259
ACACTAT66600.06.7004038
AACACTA67050.06.54808867
GTATATC147450.06.24627971
GTGTATC72050.06.0392051
GTGTATG74150.05.9659721
GTGTATT99150.05.85139941
GTGTAGG72050.05.83789831
GTACACA61450.05.78278061
GTGTACT60200.05.7823881
GTGTAAC38152.7830538E-105.70281461
GTATATT183750.05.60432771
GTATACC82400.05.4566611
GTATAGA83150.05.4074431
ACCAAAC90700.05.40055663
Produced by FastQC (version 0.11.5)