FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17150565
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACA60950.08.0093451
GTGTAAA88100.07.2778431
ACACTAT59300.07.15693148
AACACTA61450.07.14166647
CACTATA60800.06.743749
GTATACC80100.06.6402781
GTGTTCG138600.06.20318461
GTGTACG84250.06.1402261
GTACACA59100.06.040941
GTGTATC69650.05.9627921
GTATAGC82800.05.8957681
GTATACG65700.05.8776871
GTATATC150550.05.8075961
GTATCCA114000.05.68828151
GTGTATG72550.05.62401531
GTATAGT64900.05.61333851
GTGTATT96100.05.53471661
GTGTAAG41604.0563464E-105.4295551
GTGTAAC41804.6020432E-105.4035771
GTGTACT60850.05.38825131
Produced by FastQC (version 0.11.5)