FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14067834
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTAT398090.28297888644406805TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA182100.040.879269
AGAGCAC187750.039.8791438
GATCGGA195100.038.8254851
TCGGAAG237900.031.6077123
ATCGGAA260650.028.9874062
AAGAGCA262550.028.9563837
GGAAGAG299600.025.3519175
GAAGAGC305500.024.7916076
CGGAAGA343500.022.0699734
AGTCACG103850.014.0324525-29
CAGTCAC117050.012.99128225-29
CCAGTCA125500.012.39194625-29
CGAATCT117150.011.93485930-34
CTCGTAT116550.011.66244340-44
CACACGT133150.011.614099510-14
CACGTCT137650.011.23441610-14
TCTCGTA124950.011.03974740-44
ACTCCAG147900.010.73868620-24
ACACGTC155850.010.17192110-14
ACGAATC139350.010.15750130-34
Produced by FastQC (version 0.11.5)