FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12733487
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG153520.12056399005237137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA103750.011.5608051
GAGCGTC141300.08.7130449
TCGGAAG159550.07.98741153
AGAGCGT175200.07.10931878
ATCGGAA203550.07.00652
CTAGATC14450.00353188255.9790117
AAGAGCG217000.05.9721237
GATCTAC41901.4551915E-115.72521971
CGGAAGA244650.05.50333554
GATCTCA54350.05.3499861
GATCCTG91250.05.09846351
Produced by FastQC (version 0.11.5)