FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15002799
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG66100.035.662391
TGAGTGA68350.034.3455242
GAGTGAT70900.032.8021623
AGTGATG107250.022.2884434
ATGGTTG123750.019.3172938
TGGTTGA150050.016.363669
GATGGTT152750.016.356277
TAGTGTG39500.012.68400515-19
GTGATGG236300.010.7858735
TGATGGT244600.010.4786416
GTAGTGT49750.09.95496315-19
AGTGTGG74600.07.410826715-19
CTAGATC12750.05.8721004140-144
GGTAGTG94400.05.74967915-19
TGAGGTA102000.05.604684410-14
Produced by FastQC (version 0.11.5)